T7 RNA Polymerase Buffer for Supercharging Your Reaction

Researchers at Stellenbosch University have developed a proprietary buffer that enables a high-temperature (50°C) IVT process, solving a key industry challenge. Unlike standard buffers that suffer a ~40% yield loss at high temperature, this formulation maintains ~93% RNA yield with standard enzyme loads while concurrently reducing immunogenic dsRNA impurities. This dual benefit of significantly higher yield and superior purity de-risks manufacturing, lowers production costs, and enhances the safety profile of the final mRNA product.